Synthesized:Article Title: Recombinant influenza A H5N1 viruses expressing fluorescent and luminescent reporter proteins
Article Snippet: Bovine uterine epithelial cells (Cal-1, developed in house at the Virology Laboratory at AHDC) were cultured in minimal essential medium (MEM, Corning Inc., Corning, NY) supplemented with 10% fetal bovine serum (FBS) and 1X Antibiotic-Antimycotic. .. Briefly, full-length genome sequences of the HA, NA, M, NP, PA, PB1, and PB2 gene segments of the TX2/24 strain (H5N1 clade 2.3.4.4b, genotype B3.13; GISAID accession number EPI_ISL_19155861) were commercially synthesized (Twist Bioscience) and cloned into the dual-promoter influenza reverse genetics plasmid pHW2000 (kindly provided by Dr. Richard Webby, St. Jude Children’s Research Hospital) using BsmBI restriction sites (New England Biolabs). .. Briefly, full-length genome sequences of the HA, NA, M, NP, PA, PB1, and PB2 gene segments of the TX2/24 strain (H5N1 clade 2.3.4.4b, genotype B3.13; GISAID accession number EPI_ISL_19155861) were commercially synthesized (Twist Bioscience) and cloned into the dual-promoter influenza reverse genetics plasmid pHW2000 (kindly provided by Dr. Richard Webby, St. Jude Children’s Research Hospital) using BsmBI restriction sites (New England Biolabs).
Article Title: The impact of highly pathogenic avian influenza H5N1 virus infection on dairy cows.
Article Snippet: .. Briefly, full length genome sequences of PB1, PB2, PA, HA, NA, NP and M gene segments of TX2/24 strain (H5N1 clade 2.3.4.4b, genotype B3.13, GISAID accession number: EPI_ISL_19155861) were synthesized commercially (Twist Bioscience) and cloned into the dual promoter influenza reverse genetics plasmid pHW2000 (kindly provided by Dr. Richard Webby at St. Jude Children’s Research Hospital) using the BsmBI (New England Biolabs) restriction sites. ..
Article Title: H5N1 influenza virus stability and transmission risk in raw milk and cheese
Article Snippet: .. In brief, full-length genome sequences of PB1, PB2, PA, HA, NA, NP and M gene segments of TX2/24 strain (H5N1 clade 2.3.4.4b, genotype B3.13, GISAID accession number: EPI_ISL_19155861) were synthesized commercially (Twist Bioscience) and cloned into the dual promoter influenza reverse genetics plasmid pHW2000 (kindly provided by Dr. Richard Webby at St. Jude Children’s Research Hospital) using the BsmBI (New England Biolabs) restriction sites. ..
Article Title: H5N1 influenza virus stability and transmission risk in raw milk and cheese.
Article Snippet: .. In brief, full-length genome sequences of PB1, PB2, PA, HA, NA, NP and M gene segments of TX2/24 strain (H5N1 clade 2.3.4.4b, genotype B3.13, GISAID accession number: EPI_ISL_19155861) were synthesized commercially (Twist Bioscience) and cloned into the dual promoter influenza reverse genetics plasmid pHW200031 (kindly provided by Dr. Richard Webby at St. Jude Children’s Research Hospital) using the BsmBI (New England Biolabs) restriction sites. ..
Clone Assay:Article Title: Recombinant influenza A H5N1 viruses expressing fluorescent and luminescent reporter proteins
Article Snippet: Bovine uterine epithelial cells (Cal-1, developed in house at the Virology Laboratory at AHDC) were cultured in minimal essential medium (MEM, Corning Inc., Corning, NY) supplemented with 10% fetal bovine serum (FBS) and 1X Antibiotic-Antimycotic. .. Briefly, full-length genome sequences of the HA, NA, M, NP, PA, PB1, and PB2 gene segments of the TX2/24 strain (H5N1 clade 2.3.4.4b, genotype B3.13; GISAID accession number EPI_ISL_19155861) were commercially synthesized (Twist Bioscience) and cloned into the dual-promoter influenza reverse genetics plasmid pHW2000 (kindly provided by Dr. Richard Webby, St. Jude Children’s Research Hospital) using BsmBI restriction sites (New England Biolabs). .. Briefly, full-length genome sequences of the HA, NA, M, NP, PA, PB1, and PB2 gene segments of the TX2/24 strain (H5N1 clade 2.3.4.4b, genotype B3.13; GISAID accession number EPI_ISL_19155861) were commercially synthesized (Twist Bioscience) and cloned into the dual-promoter influenza reverse genetics plasmid pHW2000 (kindly provided by Dr. Richard Webby, St. Jude Children’s Research Hospital) using BsmBI restriction sites (New England Biolabs).
Article Title: The impact of highly pathogenic avian influenza H5N1 virus infection on dairy cows.
Article Snippet: .. Briefly, full length genome sequences of PB1, PB2, PA, HA, NA, NP and M gene segments of TX2/24 strain (H5N1 clade 2.3.4.4b, genotype B3.13, GISAID accession number: EPI_ISL_19155861) were synthesized commercially (Twist Bioscience) and cloned into the dual promoter influenza reverse genetics plasmid pHW2000 (kindly provided by Dr. Richard Webby at St. Jude Children’s Research Hospital) using the BsmBI (New England Biolabs) restriction sites. ..
Article Title: H5N1 influenza virus stability and transmission risk in raw milk and cheese
Article Snippet: .. In brief, full-length genome sequences of PB1, PB2, PA, HA, NA, NP and M gene segments of TX2/24 strain (H5N1 clade 2.3.4.4b, genotype B3.13, GISAID accession number: EPI_ISL_19155861) were synthesized commercially (Twist Bioscience) and cloned into the dual promoter influenza reverse genetics plasmid pHW2000 (kindly provided by Dr. Richard Webby at St. Jude Children’s Research Hospital) using the BsmBI (New England Biolabs) restriction sites. ..
Article Title: H5N1 influenza virus stability and transmission risk in raw milk and cheese.
Article Snippet: .. In brief, full-length genome sequences of PB1, PB2, PA, HA, NA, NP and M gene segments of TX2/24 strain (H5N1 clade 2.3.4.4b, genotype B3.13, GISAID accession number: EPI_ISL_19155861) were synthesized commercially (Twist Bioscience) and cloned into the dual promoter influenza reverse genetics plasmid pHW200031 (kindly provided by Dr. Richard Webby at St. Jude Children’s Research Hospital) using the BsmBI (New England Biolabs) restriction sites. ..
Plasmid Preparation:Article Title: Recombinant influenza A H5N1 viruses expressing fluorescent and luminescent reporter proteins
Article Snippet: Bovine uterine epithelial cells (Cal-1, developed in house at the Virology Laboratory at AHDC) were cultured in minimal essential medium (MEM, Corning Inc., Corning, NY) supplemented with 10% fetal bovine serum (FBS) and 1X Antibiotic-Antimycotic. .. Briefly, full-length genome sequences of the HA, NA, M, NP, PA, PB1, and PB2 gene segments of the TX2/24 strain (H5N1 clade 2.3.4.4b, genotype B3.13; GISAID accession number EPI_ISL_19155861) were commercially synthesized (Twist Bioscience) and cloned into the dual-promoter influenza reverse genetics plasmid pHW2000 (kindly provided by Dr. Richard Webby, St. Jude Children’s Research Hospital) using BsmBI restriction sites (New England Biolabs). .. Briefly, full-length genome sequences of the HA, NA, M, NP, PA, PB1, and PB2 gene segments of the TX2/24 strain (H5N1 clade 2.3.4.4b, genotype B3.13; GISAID accession number EPI_ISL_19155861) were commercially synthesized (Twist Bioscience) and cloned into the dual-promoter influenza reverse genetics plasmid pHW2000 (kindly provided by Dr. Richard Webby, St. Jude Children’s Research Hospital) using BsmBI restriction sites (New England Biolabs).
Article Title: The impact of highly pathogenic avian influenza H5N1 virus infection on dairy cows.
Article Snippet: .. Briefly, full length genome sequences of PB1, PB2, PA, HA, NA, NP and M gene segments of TX2/24 strain (H5N1 clade 2.3.4.4b, genotype B3.13, GISAID accession number: EPI_ISL_19155861) were synthesized commercially (Twist Bioscience) and cloned into the dual promoter influenza reverse genetics plasmid pHW2000 (kindly provided by Dr. Richard Webby at St. Jude Children’s Research Hospital) using the BsmBI (New England Biolabs) restriction sites. ..
Article Title: H5N1 influenza virus stability and transmission risk in raw milk and cheese
Article Snippet: .. In brief, full-length genome sequences of PB1, PB2, PA, HA, NA, NP and M gene segments of TX2/24 strain (H5N1 clade 2.3.4.4b, genotype B3.13, GISAID accession number: EPI_ISL_19155861) were synthesized commercially (Twist Bioscience) and cloned into the dual promoter influenza reverse genetics plasmid pHW2000 (kindly provided by Dr. Richard Webby at St. Jude Children’s Research Hospital) using the BsmBI (New England Biolabs) restriction sites. ..
Article Title: H5N1 influenza virus stability and transmission risk in raw milk and cheese.
Article Snippet: .. In brief, full-length genome sequences of PB1, PB2, PA, HA, NA, NP and M gene segments of TX2/24 strain (H5N1 clade 2.3.4.4b, genotype B3.13, GISAID accession number: EPI_ISL_19155861) were synthesized commercially (Twist Bioscience) and cloned into the dual promoter influenza reverse genetics plasmid pHW200031 (kindly provided by Dr. Richard Webby at St. Jude Children’s Research Hospital) using the BsmBI (New England Biolabs) restriction sites. ..
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